Investigation on effects of Hibiscus sabdariffa L. on hemoglobin level of mice with phenylhydrazine-induced anemia
DOI:
https://doi.org/10.18686/fnc270Keywords:
Hibiscus sabdariffa L.; anemia; hemoglobin; phenylhydrazine-induced anemia; iron contentAbstract
With Sub-Saharan Africa having the highest prevalence of anemia globally and inequitable access to health care, traditional medicines are used by some of the population for treatment. Hibiscus sabdariffa L. (HS) is known to have medicinal applications, including its use in the treatment of anemia. The purpose of this study was to investigate the effect of HS on the hemoglobin level in anemic mice. A total of 15 mice were divided into three groups: Group A was the control group, while Groups B and C were the experimental groups, in which anemia was induced using phenylhydrazine. Only group C received oral administration of HS at 400 mg/kg. Blood samples were collected from the tail vein and analyzed using a HemoCue hemoglobin meter. The HS iron content was assessed using an atomic absorption spectrometer. The mean iron content for HS was 6.3 ± 0.6 mg/L. No significant difference (p = 0.359) was observed in the mean hemoglobin levels between Groups A and B, which were 14.88 g/dl and 13.78 g/dl respectively. Higher hemoglobin levels were observed when Group C (17.46 g/dl) was compared with Group A (p = 0.005) and Group B (p = 0.001). These findings suggest the efficacy of HS in improving the hemoglobin level in anemic mice. Further investigations on doses and the mechanism are needed before recommendations for human consumption.
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